[Analytical methods] Pros-and-Cons-of-Three-High-Resolution-Mass-Spec-Approaches
Pros and Cons of Three High-Resolution Mass Spec Approaches Mike May Posted : June 8, 2017 Source: O'Donnell, V.B., Murphy, R.C., & Watson, S.P. (2014). Platelet lipidomics: modern day perspective on lipid discovery and characterization in platelets. Circulation research, 114 7 , 1185-203 . Mass spectrometry (MS) measures the mass ( m ) to charge ( z ) ratio— m/z —of the ions in a sample. Those ratios provide an atomic signature for what makes up a sample. In many situations, z = 1, so the ratio provides the mass of the ions. In original instruments, the mass could only be measured to single-digit units, or integers. With high-resolution mass spectrometry (HRMS), the mass can be measured to several decimal places. Often, ordinary MS is said to measure nominal mass and HRMS measures exact mass. By obtaining data that provide a more accurate representation of the mass of ions in a sample, this technique becomes usefu...